Research reference tool
Reconstitution calculator
Convert vial mg + bacteriostatic water ml into U-100 syringe units. Research reference. Not medical advice.
Visual protocol
How to reconstitute a peptide
Verified step-by-step process. Every detail matters to preserve protein integrity.
Materiales necesarios
Before starting, gather: the lyophilized peptide vial, a bacteriostatic water (BAC water) vial, U-100 insulin syringes (1 ml or 0.5 ml), and 70% alcohol swabs.
Bacteriostatic water contains 0.9% benzyl alcohol as a preservative. This is what allows safe multiple-use from the vial.
Limpiar los topes de los viales
Pasa una toallita con alcohol al 70% sobre el tope de goma de ambos viales (peptide and BAC water). Use a single sweeping motion in one direction.
Wait at least 10 seconds before inserting the needle. The alcohol needs to evaporate completely. Inserting the needle too early can introduce alcohol residue into the vial and damage the peptide.
Usa una jeringa de insulina U-100
U-100 insulin syringes are the smallest and most precise available. Recommended: 1 ml for sufficient range, or 0.5 ml for small doses.
In a U-100 syringe: 100 units = 1 ml. If your calculated dose above is, for example, 5 units, it means drawing to the 5 mark on the syringe.
Empuja aire antes de aspirar
Before drawing the BAC water, draw the same volume of aire que quieres extraer. Luego inserta la aguja en el vial invertido and push that air in.
This step equalizes the pressure inside the vial and lets the water flow easily into the syringe without needing to create excessive vacuum.
Aspirar agua bacteriostática
With the BAC vial inverted, slowly pull the plunger to draw the desired deseada de agua. Se recomiendan 2 ml por vial de péptido, making dosing easier without making doses too small.
Between 1 ml and 3 ml is acceptable. More water makes micro-dosing easier. 2 ml gives a good balance of concentration and precision.
Inyecta contra la pared, no sobre el péptido
Mientras inyectas, mueve la punta de la aguja hacia la pared interior del vial. Inyecta el agua BAC contra el cristal. El agua rebota y resbala gently to the bottom where the peptide is. Never direct the stream directly onto the powder.
The impact of water against the glass slows it down: it reaches the peptide slowly without breaking its bonds. A direct stream onto the powder applies mechanical force that denatures the protein: irreversible damage.
Nunca agitar, solo rodar
Nunca sacudas el vial. Agitar crea espuma, introduce burbujas of air and creates stress at air-liquid interfaces where peptides denature and aggregate irreversibly.
Instead, gently roll the vial between your palms or make slow circles. This mixes the content without damaging the protein structure.
Esperar la disolución completa
Let the vial rest at room temperature for 15–30 minutes, gently swirling occasionally. The solution should become completely clear, with no lumps or visible particles.
If after 30 minutes there are undissolved particles, it may indicate peptide degradation or solvent incompatibility. Do not use.
Aspirar la dosis correcta
Invert the reconstituted vial and insert the needle. Pull the plunger to the number of U-100 units indicated by the calculator above. Check that there are no air bubbles in the syringe.
If there are bubbles, hold the syringe with the needle up and tap gently with your finger to make them rise, then carefully expel the air before injecting.
Almacenar en refrigerador
Store the reconstituted vial in the refrigerator at 2–8°C, away from direct light. Bacteriostatic water maintains sterility for 28 días. Después de ese plazo descartar el vial.
Never freeze a reconstituted vial: ice crystals break the protein structure. Lyophilized (unreconstituted) peptides can be frozen for up to 24 months.
⚕ Educational reference, not medical advice. Verified with multiple reconstitution protocol sources. Always follow your doctor's instructions.